Practical Course: High resolution microscopy with fungi
The kingdom of fungi includes single cell yeasts and multicellular organisms, producing distinguishable tissues. Therefore, they can represent a link between microbiology and cellular biology. Fungi are used for research on cellular growth, transportation processes, mating, and intreacellular signal transduction. Basidiomycetes are investigated for pheromone recognition, fruiting body production, or excretion processes. The new microscopical high resolution techniques allow for deeper insights into fungal structures and regulatory mechanisms.
The course will present several advanced microscopic techniques, e.g. fluorescence based localization of intracellular organelles, co-localization of proteins, and an introduction in superresolution microscopy. The examination of signal molecules will show their direct impact on physiological and biochemical changes in the analyzed fungi. Further, 3D reconstruction of cellular structures and long-term observations will be introduced. The CLSM-780 microscope with SR-SIM module that is to be used for multichannel fluorescence detection with resolution of 100 nm. Fluorescence correlation spectroscopy (FCS) and Fluorescence Dynamics Photomanipulation experiments offer new possibilities for the investigation not only of fungi, but also for other microorganisms.
The number of participants will be limited to 15 in order to assure a workshop environment conducive to “hand to hand” exercises.
Students only need a basic background in microscopy.
Registration
Email to katja.praefcke@jsmc.info
Date and Time
August 24, 2015 - August 25, 2015 // 09:00 - 16:00
Location
Location: Neugasse 25, Seminar room
