Investigation of the stress response system of A. fumigatus by redox proteomics
It has been shown that regulation of the redox homeostasis is an important trait for pathogenic microorganisms to survive in the host. To gain more insights into the mechanisms how A. fumigatus maintains redox homeostasis and to learn which proteins are specifically modified under redox imbalance, we aim to characterize the changes of the redox proteome of A. fumigatus under several in vitro stress conditions. To study the impact of reactive oxygen (ROS) and nitrogen species (RNS) on post-translation modification of proteins involved in the stress response system of A. fumigatus 2D-gel electrophoresis (2-DE) will be used. Furthermore, we will analyze the impact of hypoxia at the redox homeostasis of A. fumigatus. Overall, the aim of the project is to identify and characterize redox-based changes in the proteome of A. fumigatus under stress oxidative conditions as well as to elucidate significance of these changes on shaping the fungal adaptome.
(2019) Redox Proteomic Analysis Reveals Oxidative Modifications of Proteins by Increased Levels of Intracellular Reactive Oxygen Species during Hypoxia Adaptation of Aspergillus fumigatus. Proteomics 19(5), e1800339.
(2017) Induction of Mitochondrial Reactive Oxygen Species Production by Itraconazole, Terbinafine, and Amphotericin B as a Mode of Action against Aspergillus fumigatus. Antimicrob Agents Chemother 61(11),
(2015) Insights into the cellular responses to hypoxia in filamentous fungi. Curr Genet 61(3), 441-455.
Start of PhD
May 1, 2013
November 30, 2017