Obtaining deeper insights into microbiome diversity using a simple method to block host and nontargets in amplicon sequencing.
(2021) Obtaining deeper insights into microbiome diversity using a simple method to block host and nontargets in amplicon sequencing. Mol Ecol Resour 21(6), 1952-1965. PubMed
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Community resource availability as a driver of convergent microbe-microbe interaction types and microbial community structures
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Abstract
Profiling diverse microbiomes is revolutionizing our understanding of biological mechanisms and ecologically relevant problems, including metaorganism (host + microbiome) assembly, functions and adaptation. Amplicon sequencing of multiple conserved, phylogenetically informative loci has therefore become an instrumental tool for many researchers. Investigations in many systems are hindered, however, since essential sequencing depth can be lost by amplification of nontarget DNA from hosts or overabundant microorganisms. Here, we introduce
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doi: 10.1111/1755-0998.13408 PMID: 33905604
