Twofold polyketide branching by a stereoselective enzymatic Michael addition.
(2015) Twofold polyketide branching by a stereoselective enzymatic Michael addition. Chem Commun (Camb) 51(48), 9872-9875.
Biochemical characterisation of bacterial terpene cyclases
The versatility of the branching module of the rhizoxin polyketide synthase was tested in an in vitro enzyme assay with a polyketide mimic and branched (di)methylmalonyl-CoA extender units. Comparison of the products with synthetic reference compounds revealed that the module is able to stereoselectively introduce two branches in one step by a Michael addition-lactonisation sequence, thus expanding the scope of previously studied PKS systems.
doi: 10.1039/c5cc03085d PMID: 25994388