Twofold polyketide branching by a stereoselective enzymatic Michael addition.

Heine D, Sundaram S, Bretschneider T, Hertweck C (2015) Twofold polyketide branching by a stereoselective enzymatic Michael addition. Chem Commun (Camb) 51(48), 9872-9875. PubMed

ILRS Authors

Christian Hertweck Srividhya Sundaram

Projects

Biochemical characterisation of bacterial terpene cyclases
Details

Abstract

The versatility of the branching module of the rhizoxin polyketide synthase was tested in an in vitro enzyme assay with a polyketide mimic and branched (di)methylmalonyl-CoA extender units. Comparison of the products with synthetic reference compounds revealed that the module is able to stereoselectively introduce two branches in one step by a Michael addition-lactonisation sequence, thus expanding the scope of previously studied PKS systems.

Identifier

doi: 10.1039/c5cc03085d PMID: 25994388

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